Selected Journal Publications

  1. S. Park, Y. Zhang, S. Lin, T.H. Wang and S. Yang, "Advances in Microfluidic PCR for Point-of-Care Infectious Disease Diagnostics", Biotechnology Advances, (accepted)
  2. S. Park, Y. Zhang, T.H. Wang and S. Yang, "Continuous Dielectrophoretic Bacterial Separation and Concentration from Physiological Media of High Conductivity",Lab on a Chip, (accepted)
  3. K.J. Liu, T.D. Rane, Y. Zhang and T.H. Wang, "Single-Molecule Analysis Enables Free Solution Hydrodynamic Separation Using Yoctomole Levels of DNA", Journal of the American Chemical Society, 133(16), 6898-6901, 2011
  4. Y. Zhang, S. Park, K. Liu, J. Tsuan, S. Yang and T.H. Wang, "A Surface Topography Assisted Droplet Manipulation Platform for Biomarker Detection and Pathogen Identification", Lab on a Chip, 11(3), 398-406, 2011
  5. Y. Zhang, S. Park, S. Yang and T.H. Wang, "An All-In-One Microfluidic Device for Parallel DNA Extraction and Gene Analysis", Biomedical Microdevices 12(6), 1043-1049, 2010
  6. K.J. Liu, M.V. Brock, I.M. Shih and T.H. Wang,"Decoding Circulating Nucleic Acids in Human Serum Using Microfluidic Single Molecule Spectroscopy", Journal of the American Chemical Society, 132(16), 5793-5798, 2010
  7. V.J. Bailey, Y. Zhang, B.P. Keeley, C. Yin, K.L. Pelosky , M. Brock , S.B. Baylin, J.G. Herman, T.H. Wang, "Single-Tube Analysis of DNA Methylation with Silica Superparamagnetic Beads", Clinical Chemistry, 56(6), 1022-1025, 2010
  8. V.J. Bailey, B.P. Keeley, C.R. Razavi, E. Griffiths, H.E. Carraway, and T.H. Wang, "DNA methylation detection using MS-qFRET, a quantum dot-based nanoassay" Methods, 52(3), 237-241, 2010
  9. T.H. Wang and P.K. Wong, "Transforming Microfluidics into Laboratory Automation" Journal of the Association for Laboratory Automation, 15(3), A15-A16, 2010
  10. X. Jiang, Y. Zheng, H.H. Chen, K.M. Leong, T.H. Wang, and H.Q. Mao, "Dual-sensitive Micellar Nanoparticles Regulate DNA Unpacking and Enhance Gene Delivery Efficiency", Advanced Materials, 22(23):2556-2560, 2010
  11. T.H. Wang, V.J. Bailey, Y. Zhang and K.J. Liu, "Quantum Dots DNA Nanosensors - Ultrasensitive Platform for Detecting Genomic Cancer Markers", BIOforun Europe, 1-2:28-30, 2010
  12. T.D. Rane, C.M. Puleo, K.J. Liu, Y. Zhang, A.P. Lee and T.H. Wang. "Counting single molecules in sub-nanolitre droplets ", Lab on a Chip, 10(2):161-164, 2010
  13. V.J. Bailey, B.P. Keeley, Y. Zhang, Y.P. Ho, H. Easwaran, M.V. Brock, K.L. Pelosky, H. E. Carraway, S. B. Baylin, J.G. Herman, and T.H. Wang . "Enzymatic Incorporation of Multiple Dyes for Increased Sensitivity in QD-FRET Sensing for DNA Methylation Detection", ChemBioChem, 11(1):71-74, 2010
  14. D.D. Nalayanda, Q Wang, W.B. Fulton, T.H. Wang and F. Abdullah, " Engineering an Artificial Alveolar-Capillary Membrane: A Novel Continuously-Perfused Model within Microchannels", Journal of Pediatric Surgery, 45(1):45-51, 2010
  15. C.M. Puleo, W.M. Ambrose, T. Takezawa, J. Elisseeff, T.H. Wang. "Integration and Application of Vitrified Collagen in Multilayered Microfluidic Devices for Corneal Microtissue Culture", Lab on a Chip, 9(22):3221-3227, 2009
  16. V. J. Bailey, H. Easwaran, Y. Zhang, E. Griffiths, S.A. Belinsky, J.G. Herman, S.B. Baylin, H.E. Carraway, T.H. Wang, "MS-qFRET: A Quantum Dot-based Method for Analysis of DNA Methylation", Genome Research, 19(8):1455-1461, 2009
  17. Y. Zhang, V. Bailey, C.M. Puleo, H. Easwaran, E. Griffiths, J.G. Herman, S.B. Baylin, T.H. Wang, "DNA Methylation Analysis on a Droplet-in-Oil PCR Array", Lab on a Chip, 9:1059-1064, 2009
  18. C.M. Puleo and T.H. Wang, "Microfluidic Means of Achieving Attomolar Detection Limits with Molecular Beacon Probes", Lab on a Chip, 9:1065-1072, 2009
  19. D. D Nalayanda, C. M. Puleo; W. B. Fulton; L.M. Sharpe, T.-H. Wang and F. Abdullah," An open-access microfluidic model for lung-specific functional studies at an air-liquid interface", Biomedical Microdevices, 11(5):1081-1089, 2009
  20. Ho, Y.P., H.H. Chen, K.W. Leong, T.H. Wang. "Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time", Journal of Visualized Experiments (JoVE), 30. http://www.jove.com/index/details.stp?id=1432, doi: 10.3791/1432, 2009
  21. H.H. Chen, Y.P. Ho, X. Jiang, H.Q. Mao, T.H. Wang and K.W. Leong, "Simultaneous Non-invasive Analysis of  DNA Condensation and Stability by Two-step QD-FRET", Nano Today, 4(2):125-134, 2009
  22. Y.P. Ho, H.H. Chen, K.W. Leong and T.H. Wang, "The convergence of Quantum-Dot-Mediated Fluorescence Resonance Energy Transfer  and Microfluidics for Monitoring DNA Polyplex Self-Assembly in Real-Time", Nanotechnology, 20(9): 095103, 2009
  23. K. Liu and T.H. Wang. " Cylindrical Illumination Confocal Spectroscopy Rectifying the Limitations of Confocal Single Molecule Spectroscopy through 1-D Beam Shaping ". Biophysical Journal, 95(6):2964-2975, 2008
  24. H.C. Yeh, C.M. Puleo, Y.P. Ho, V.J. Bailey, T.C. Lim, K. Liu and T.H. Wang. " Tunable Blinking Kinetics of Cy5 for Precise DNA Quantification and Single-Nucleotide Difference Detection ". Biophysical Journal, 95(2):729-737, 2008
  25. K. Liu, Y. Zhang and T.H. Wang. " Application of Quantum Dots for Analysis of Biomolecules ". IEEE NANO Magazine, 2(1):14-18, 2008
  26. C.M. Puleo, H.C. Yeh and T.H. Wang. " Coupling Confocal Fluorescence Detection and Recirculating Microfluidic Control for Single Particle Analysis in Discrete Nanoliter Volumes ". Lab on a Chip, 8:822-825, 2008
  27. T.C. Lim, V.J. Bailey, Y.P. Ho and T. H. Wang. " Intercalating dye as an acceptor in quantum dot mediated FRET ". Nanotechnology, 19(7):075701, 2008
  28. H.H. Chen, Y.P. Ho, X. Jiang, H.Q. Mao, T.H. Wang and K.W. Leong. " Quantitative Comparison of Intracellular Unpacking Kinetics of Polyplexes by a Model Constructed from Quantum Dot-FRET ". Molecular Therapy, 16(2):324-332, 2008
  29. C.M. Puleo , H.C. Yeh and T.H. Wang. " Applications of MEMS Technologies in Tissue Engineering ". Tissue Engineering, 13(12): 2839-2854, 2007
  30. S.Y Chao, Y.P. Ho, V. Bailey and T.H. Wang. " Quantification of Low Concentration DNA Using Single Molecule Detection and Velocity Measurement in a Microchannel ". Journal of Fluorescence, 17(6):767-774, 2007
  31. D.D. Nalayanda, C.M. Puleo, W.B. Fulton, T.H. Wang and F. Abdullah, " Characterization of Pulmonary Cell Growth Parameters in a Continuous Perfusion Microfluidic Environment". Experimental Lung Research, 33(6):321-335, 2007
  32. H. C. Yeh, C. Puleo, T. C. Lim, Y. P. Ho, P. Giza, R. C. Huang, and T. H. Wang. "A Microfluidic-FCS platform for investigation on the dissociation of Sp1-DNA complex by doxorubicin". Nucleic Acids Research, 34(21):e144, 2006
  33. Y. P. Ho, H. H. Chen, K. W. Leong, T. H. Wang. "Evaluating the Intracellular Stability and Unpacking of DNA Nanocomplexes by Quantum Dots-FRET". Journal of Controlled Release, 116:83-89, 2006
  34. C. M. Puleo, K. Liu, T. H. Wang, "Pushing miRNA quantification to the limits: high-throughput miRNA gene expression analysis using single-molecule detection," Nanomedicine, 1(1):123-127, 2006
  35. H. C. Yeh, Y. P. Ho, I. M. Shih, T. H. Wang, " Homogeneous point mutation detection by quantum dot-mediated two-color fluorescence coincidence analysis," Nucleic Acids Research, 34(5):e35, 2006
  36. C. Y. Zhang, H. C. Yeh, M. Kuroki, T. H. Wang, " Single-Quantum-Dot-Based DNA Nanosensor," Nature Materials, 4(11):826-831, 2005
  37. Y. P. Ho, M. C. Kung, S. Yang, T. H. Wang, " Multiplexed Hybridization Detection with Multicolor Colocalization of Quantum Dot Nanoprobes," Nano Letters, 5(9): 1693-1697, 2005
  38. H. C. Yeh, S. Y. Chao, Y. P. Ho, T. H. Wang," Single-Molecule Detection and Probe Strategies for Rapid and Ultrasensitive Genomic Detection," Current Pharmaceutical Biotechnology, 6(6):453-461, 2005
  39. T. H. Wang, Y. H. Peng, C. Y. Zhang, P. K. Wong, C. M. Ho, " Single-molecule tracing on a fluidic microchip for quantitative detection of low-abundance nucleic acids," Journal of the American Chemical Society, 127 (15): 5354-5359 2005 (Highlighted in Nanozone news in Nature, April 28, 2005)
  40. C. Y. Zhang, S. Y. Chao., T. H. Wang, " Comparative quantification of nucleic acids using single-molecule detection and molecular beacons," Analyst, 130 (4): 483-488 2005
  41. H. C. Yeh, Y. P. Ho, T. H. Wang, " Quantum dot?mediated biosensing assays for specific nucleic acid detection," Nanomedicine: Biol. Med., 1 (2): 115-121 2005
  42. P. K. Wong, C. Y. Chen, T. H. Wang, C. M. Ho, " Electrokinetic bioprocessor for concentrating cells and molecules," Analytical Chemistry, 76 (23): 6908-6914 2004
  43. P. K. Wong, T. H. Wang, J. H. Deval, C. M. Ho, " Electrokinetics in micro devices for biotechnology applications," IEEE-ASME Transactions on mechatronics, 9 (2): 366-376 2004

Selected Conference Publications

  1. H. H. Chen, Y. P. Ho, T. H. Wang, K. W. Leong, " Intracellular Trafficking of QD-FRET Nanoparticles for Gene Delivery," Biomedical Engineering Society 2005 (accepted)
  2. Y. P. Ho, M. C. Kung, T. H. Wang, " Separation-free Detection of Low-abundant Biomolecules with Two-Color Colocalization of Quantum Dot Probes," Ninth International Conference on Miniaturized Chemical and Biochemical Analysis Systems (MicroTAS 2005) 2005 (accepted)
  3. H. C. Yeh, Y. P. Ho, T. H. Wang, " Quantum Dot-Mediated Separation-Free Assay for Single Nucleotide Polymorphism (SNP) Detection," NSTI- Bio-Nanotechnology Conference and Trade Show 2005
  4. H. C. Yeh, E. Simone, C. Y. Zhang, T. H. Wang, " Single Bio-molecule Detection with Quantum Dots in a Microchannel," 17th IEEE International Conference on Micro Electro Mechanical Systems (MEMS 2004) 371-374 2004
  5. S. Y. Chao, C. Y. Zhang, T. H. Wang, " Measurement of in-situ Flow Velocity Using Single-Molecule Detection for the Application of Biomolecule Quantification," 2004 Solid-State Sensor, Actuator, and Microsystems Workshop (Hilton Head 2004) 176-179 2004
  6. T. H. Wang, C. M. Ho, " Nano/micro Technologies for Single Molecule Manipulation and Detection," 2003 IEEE international Conference on Robotics and Control 2003
  7. P. K. Wong, C.Y. Chen, T.H. Wang, C.M. Ho, " An AC Electroosmotic Processor for Biomolecules," The 12th International Conference on Solid-state Sensors, Actuators, and Microsystems, Transducers'03 2003
  8. T. H. Wang, C. M. Ho, " Nano/Micro Technologies for Single Molecule Manipulation and Detection," IEEE 2004 International Conference on Robotics and Automation 2003
  9. T. H. Wang, P. K. Wong, C.M. Ho, " Electrical Molecular Focusing for Laser Induced Fluorescence Based Single DNA Detection," The 15th IEEE International Conference on Micro Electro Mechanical Systems (MEMS2002) 15-18 2002
  10. P. K. Wong, T. H. Wang, C. M. Ho, " Formation of Optical Fiber Tip by Surface Tension Controlled Etching," 2002 Solid-State Sensor, Actuator, and Microsystems Workshop (Hilton Head 2002) 2002
  11. T. H. Wang, C. M. Ho, " A Zepto Mole DNA Micro Sensor," The 14th IEEE International Conference on Micro Electro Mechanical Systems (MEMS2001) 431-434 2001
  12. T. H. Wang, S. Masset, C. M. Ho, " Molecular Beacon Based Biological Detection System," The International Conference on Mathematics and Engineering techniques in Medicine and Biological Sciences (METMBS 00') 2000

Book Chapters

  1. T. H. Wang, C. M. Ho, " Nano/Micro Technologies for Detecting a Single DNA Molecule," Frontiers in Biomedical Engineering Kluwer Academic/Plenum Publishers 2003

Patents

  1. T. H. Wang, Y. P. Ho, " Fluorescence Self-amplification Facilitated by Molecular Nanoprobes and Micro Concentration Devices for Rapid and Ultrasensitive Biological Detection," 2004 (U.S. provisional patent)
  2. T. H. Wang, C. Y. Zhang, " Semiconductor quantum-dot based inorganic/organic hybrid biosensor for homogenous separation-free detection of biomolecules," 2004 (U.S. provisional patent)
  3. C. M. Ho, J. Liao, T. H. Wang, " Ultrasensitive Molecule Detection of Urinary Tract Infection," (U.S. Patent Pending, UC Case No. 2001-249)
  4. C. M. Ho, J. Miller, J. Huang, T. H. Wang, M. Liu, " Electrochemical Detection of Mismatch in Nucleic Acids (EDEMNA)," (U.S. Patent Pending, UC Case No. 2001-158-1)
  5. C. M. Ho, T. H. Wang, " Electronics Integrated With Bio-Reactor/Channel For Detection Or Fabrication Of Bio-Materials," (U.S. Patent Pending, UC Case No. 2000-499 )
  6. T. H. Wang, " Method for determining standard cycle time of a stage dynamically," U.S. Patent US5825650 1997